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doi: 10.1038/s41598-021-86425-6 [DOI] [PMC free article] [PubMed] [Google Scholar] 193

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Briefly, HaCaT keratinocytes were seeded at a density of 10,000 cells per well in 200 μl fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 °C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment