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Between Fcμ5 and β-hairpin 2, Q81 J can form a hydrogen bond with the carbonyl group of F358 Cμ3-5B , and several interactions occur between residues T84-Q87 on J chain and N545-R550 on Cμ4-5B including a van der Waals contact between A85 J to V547 Cμ4-5B (Fig