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Therefore, by activating this NCOA4-mediated ferritinophagy pathway, Erastin promotes the release of free iron, which in turn amplifies ROS production and lipid peroxidation, resulting in an autophagy-dependent mode of ferroptosis

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50 mM phosphate buffer (pH 6.1), 16 mM guaiacol, 2 mM H 2 O 2 and 0.1 mL enzyme extract were mixed in the reaction mixture