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Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a multifunctional cytokine that plays critical roles in immune modulation, serving as a bridge between hematopoiesis and immune activation ( GM-CSF enhances the survival, proliferation, and differentiation of myeloid lineage cells such as neutrophils, macrophages, and dendritic cells (DCs) ( Oncolytic viruses (OVs) armed with GM-CSF lead to localized cytokine expression within the tumor microenvironment while enhancing tumor cell susceptibility to viral infection by driving these cells into the cell cycle