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Early research has shown that proinflammatory cytokines (e.g., tumor necrosis factor-α [TNF-α] and interferon-γ) impair TJ integrity ( Regulatory Mechanisms of the TJ Barrier The innate immune system can sense pathogen-associated molecular patterns via pattern recognition receptors, including Toll-like receptors (TLRs) and nucleotide-binding oligomerization domain-containing proteins (i.e., NOD1 and NOD2) ( in vitro ( in vitro and in vivo (46, Endogenous machinery to suppress TJs is regulated by zonulin, a eukaryotic analog of the ZO toxin produced by Vibrio cholerae ( ex vivo (50)

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