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Briefly, 2 × 10 7 cells were lysed in 600 μl of PLB buffer (100 mM KCl, 5 mM MgCl 2 , 10 mM HEPES pH 7.0 and 0.5% NP-40) with RNaseOUT recombinant ribonuclease inhibitor (Invitrogen, 10777019) and protease inhibitor (APExBIO, k1007) and 12 μl of lysate was saved as input

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At the same time, many researchers have shown that TMAO has the potential as a biomarker for CKD and a target for diagnosis and treatment (Tomlinson and Wheeler, 2017)