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The role of metabolic reprogramming in tumor cells has now gained attention with the realization that oncogenic mutations in isocitrate dehydrogenase (IDH) 1 and 2 have important consequences in establishing metabolic phenotypes in glioma cells [145]

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The luciferin formed is detected in a coupled reaction using Ultra-Glo™ Recombinant Luciferase that generates a glow-type luminescence that is proportional to the amount of glutathione present in cells