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It is the core citation behind all downstream NNMT-inhibitor drug discovery, including 5-Amino-1MQ — but it used a genetic approach (ASO), not a small-molecule inhibitor, and it was conducted in rodents

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Advanced analytical techniques are enabling researchers to track the compound's effects on global metabolite profiles, revealing complex interactions between NAD+ metabolism, methylation cycles, and other metabolic pathways that were previously unappreciated