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For the quantification of these metabolites in TIF samples, 5 µl samples were extracted in 45 µl ice-cold extraction solvent (75% acetonitrile, 25% methanol and 0.1% formic acid) containing isotope-labelled internal standards (36 µM 13 C 2 , 15 N-GSH, 0.92 µM 13 C 4 , 15 N 2 -GSSG and 10 µM D 4 -cystine)