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The accumulation of intracellular ferrous iron (Fe 2+ ) can lead to the generation of highly reactive hydroxyl radicals (●OH), one of the most harmful reactive oxygen species (ROS) in biological systems (Rochette et al., 2022) via the Fenton reaction, as shown below

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To achieve smaller particle sizes, the dispersion underwent ultrasonic probe sonication for 5 min at a 30% pulse rate ( TABLE 1 Formulation design parameters for the optimization of Quercetin-loaded niosomes using box-behnken design