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Although some genes that were typically associated with ISR and cholesterol and FA biosynthesis were also upregulated and downregulated, respectively, in Cse −/− mice on a no-Trp diet in comparison to Cse +/− and Cse −/− mice on the control diet, their induction on the no-Cys diet was significantly more pronounced, underscoring a special role of cysteine in the regulation of ISR and fat metabolism (Fig