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The charged residues of peptide (such as Arg/Lys) form a salt bridge with the acidic residues on the enzyme surface (Glu256, Asp322), which induces the conformational change of the active center and destroys the stable coordination of copper ion (Deri et al., 2016)

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IGF-1R, the insulin receptor and the receptor for IGF-2 sit in an overlapping ligand space, and because both receptors are assembled from half-receptors in the same compartment, cells expressing both will also display IR/IGF-1R hybrid receptors composed of one alpha-beta half of each