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Analysis of gene transcription levels Total RNA was extracted from tissue or cells using RNeasy Mini/Micro Kit (Qiagen), treated with RNase-Free DNase Set (Qiagen), and reverse transcribed using the High Capacity RNA-to-cDNA Kit (Applied Biosystems) according to the manufacturers’ protocols

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For most researchers, 3 mL (10 mg/mL) offers the best balance of precision and comfort